日本性感染症学会誌 Journal of Japanese Society for Sexually Transmitted Infections

Online ISSN: 2434-2505　Print ISSN: 0917-0324
日本性感染症学会 Japanese Society for Sexually Transmitted Infections
〒162-0801東京都新宿区山吹町358-5アカデミーセンター Japanese Society for Sexually Transmitted Infections　Academy Center, 358-5 Yamabuki-cho, Shinju-ku, Tokyo 162-0801, Japan
Japanese Journal of Sexually Transmitted Infections 31(1): 85-89 (2020)
doi:10.24775/jjsti.O-2020-0009

原著原著

女性の初尿検体と導尿検体におけるHPV検出についての検討Comparison of human papillomavirus detection in first-catch urine and urine collected by catheterization

川口 昌平1，重原 一慶1，大阪 康宏2，中川 朋美1，加藤 佑樹1，坂本 次郎1，中嶋 一史3，笹川 寿之2，溝上 敦1Shohei Kawaguchi1, Kazuyoshi Shigehara1, Yasuhiro Osaka2, Tomomi Nakagawa1, Yuki Kato1, Jiro Sakamoto1, Kazufumi Nakashima3, Toshiyuki Sasagawa2, Atsushi Mizokami1

1 金沢大学医薬保健研究域医学系集学的治療学（泌尿器科学）Department of Integrative Cancer Therapy and Urology, Kanazawa University Graduate School of Medical Science

2 金沢医科大学生殖周産期医学（産婦人科）Department of Reproductive and Perinatal Medicine, Kanazawa Medical University

3 中嶋医院Nakashima Clinic

受付日：2020年6月18日Received: June 18, 2020
受理日：2020年9月16日Accepted: September 16, 2020
発行日：2020年11月8日Published: November 8, 2020
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【目的】尿が女性のHPV感染を調査するのに良好で非侵襲的なツールになる可能性が示唆されている。今回、我々は尿中HPVについて女性の初尿検体と導尿検体における検出率を調査した。

【方法】産科・婦人科疾患で手術を受ける患者を対象とし、術前の初尿検体及び導尿検体を採取した。尿沈渣を液状細胞診用保存液に保存し、それぞれの検体についてnested-PCR法を用いてHPV-DNAの有無を調査した。

【結果】対象者は49例で、平均年齢は47.7歳（22–74歳）であった。有効検体48例中、初尿検体の5例（10.4%）でHPV陽性であり、導尿検体の2例（4.2%）でHPV陽性であった。

【考察】導尿検体でもHPVが検出されていることから、初尿検体中のHPVの一部は尿路のHPV感染をみているものと考えられた。尿中HPVは採取法により検出率が変わる可能性もあり、更なる研究が必要であると考えられた。

Objective: Human papillomavirus (HPV) is known to cause cervical cancer in women. A comparison of HPV tests using cervical samples and first-catch urine samples reported a high concordance rate, suggesting that urine may be a good, noninvasive tool for screening HPV infection. We investigated the detection of HPV in first-catch urine and urine collected by catheterization to determine whether HPV in first-catch urine is actually infected in the urinary tract or if vulvar contamination occurs.

Methods: We collected first-catch urine and urine collected by catheterization. The urine sediment was preserved in a preservation solution for liquid cytology. Then, the prevalence of HPV deoxyribonucleic acid was investigated using the nested polymerase chain reaction method.

Results: Forty-nine patients with an average age of 47.7 years (range, 22–74 y) were included in this study. Of 48 effective specimens (β-globin positive), 5 (10.4%) of the first-catch urine specimens and 2 (4.2%) of the urine specimens collected by catheterization were found to be HPV positive.

Discussion: The HPV positive rate was higher in the first-catch urine than in the urine collected by catheterization, but no significant difference was observed. Because HPV was also detected in the urine collected by catheterization, it is believed that some of the HPV detected in the first-catch urine was the result of HPV infection in the urinary tract. Urine sampling, which is noninvasive and easy to collect, appears to be a good tool for assessing HPV infection in women. However, the detection rate may vary depending on collection method. Further studies are needed.

Key words: cervical cancer; human papillomavirus; urine

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