日本性感染症学会誌 Journal of Japanese Society for Sexually Transmitted Infections

Online ISSN: 2434-2505 Print ISSN: 0917-0324
日本性感染症学会 Japanese Society for Sexually Transmitted Infections
〒162-0801東京都新宿区山吹町358-5アカデミーセンター Japanese Society for Sexually Transmitted Infections Academy Center, 358-5 Yamabuki-cho, Shinju-ku, Tokyo 162-0801, Japan
Japanese Journal of Sexually Transmitted Infections 31(1): 67-74 (2020)
doi:10.24775/jjsti.O-2020-0001

原著原著

日本人男性不妊患者の尿および精液検体からのHPV, Mycoplasma, Ureaplasma検出についての検討Assessment of human papillomavirus, Ureaplasma spp., Mycoplasma genitalium, and Mycoplasma hominis in semen and first-void urine specimens of male partners of infertile couples

金沢大学大学院医薬保健学総合研究科泌尿器集学的治療学Department of Integrative Cancer Therapy and Urology Kanazawa University Graduate School of Medicical Science

受付日:2020年3月4日Received: March 4, 2020
受理日:2020年7月9日Accepted: July 9, 2020
発行日:2020年11月7日Published: November 7, 2020
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当研究では1年以上の不妊を主訴に受診した日本人男性患者(77人)における初尿と精液からDNAを採取しPCRを用いてhuman papillomavirus(HPV), Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma spp.の有無を測定した。尿または精液検体からのそれぞれのDNA検出率は6.49%(n=5), 6.49%(n=5), 3.90%(n=3), 14.3%(n=11)であった。M. hominis, Ureaplasma spp.およびHPVの精液と対応する尿検体からの検出との間には、完全な一致(カッパ係数(K)=1.00)と高い一致(K=0.673)(K=0.737)を認めた。非無精子症患者(55人)においてHPV陽性群(3人)は陰性群(52人)よりも精子濃度および精子運動率が有意に低かった(P=0.0261)(P=0.0328).

The cause of male infertility may be related to genital infections, such as human papillomavirus (HPV), Mycoplasma, or Ureaplasma in 8%–35% of the cases. We collected DNA from first-void urine and semen specimens from male partners of infertile couples (n=77) and screened the samples for HPV, Mycoplasma genitalium, Mycoplasma hominis, and Ureaplasma spp. by polymerase chain reaction. Among 77 patients, the DNA detection rates of HPV, M. genitalium, M. hominis and Ureaplasma spp. from first-void urine or semen samples were 6.49% (n=5), 6.49% (n=5), 3.90% (n=3), and 14.30% (n=11), respectively. Strong agreement between semen and corresponding urine specimens was identified with regard to the detection of M. hominis, Ureaplasma spp., and HPV, (kappa coefficient [K]=1.0, 0.673, and 0.737, respectively). No significant difference in DNA detection rates was observed between patients with azoospermia (n=22) and those without (n=55). However, non-azoospermia patients with PCR-confirmed HPV infection (n=3) had significantly lower sperm concentration (median, 2.00×106/ml vs. 20.6×106/ml; P=0.0261) and sperm motility (median 13.0% vs. 50.0%; P=0.0328) than HPV PCR-negative patients (n=52). In asymptomatic infertile men, HPV in semen may affect sperm concentration and sperm motility.

Key words: infertile man; HPV; Mycoplasma; Ureaplasma

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